Moving folders to 2nd Drive

Redhat Enterprise 6 32bit. I'm a noob so I do not know all the Linux terms. We want to create two partitions on one drive. We also want to move folders such as /home /var /temp etc. to the 2nd partition. Now will the programs still work if we wiped out the 1st partition and installed the same OS with updated patches from an image? Basically what it is, is we have two computers. Only one has access to the Internet. We want to make an image of the fully patched machine and transfer it to the standalone machine without having to reinstall all the software. If this is possible what folders do we need to move to the 2nd partition? Also what size do you think we should make the 1st partition if most of the files will go to the 2nd partition?